Hematology, Transfusion and Cell Therapy, Vol. 43, Issue 1, January-March 2021

Original article 1
Autologous blood salvage in cardiac surgery: clinical evaluation, efficacy and levels of residual heparin 1
Methodology 2
Patients 2
Cell saver procedure 2
Sample collection 3
Laboratory analysis of samples 4
Statistical analysis 4
Results 4
Discussion 5
Conflicts of interest 7
References 7
Allergic reactions to asparaginase: retrospective cohort study in pediatric patients with acute lymphoid leukemia 9
Introduction 10
Method 10
Statistical analysis 10
Results 10
Discussion 12
Conclusion 13
Conflicts of interest 13
Appendix A Supplementary data 13
References 13
Yttrium-90 radiosynovectomy in knees and ankles (25 joints in 22 hemophilic patients). Short-term results 15
Introduction 16
Methods 16
Clinical evaluation 16
Isotope injection 16
Results 17
Discussion 17
Conclusion 19
Conflicts of interest 19
References 20
Follow-up and outcome of the twelve-year experience in adult patients with acute promyelocytic leukemia 21
Introduction 21
Material and methods 22
Patients and diagnostic procedures 22
Treatment data 22
Statistical analysis 22
Results 23
Patient characteristics 23
Clinical outcome 23
Discussion 23
Conflicts of interest 26
Acknowledgments 26
References 26
Ultraconserved long non-coding RNA uc.112 is highly expressed in childhood T versus B-cell acute lymphoblastic leukemia 28
Introduction 29
Materials and methods 29
Patients 29
RNA extraction, complementary deoxyribonucleic acid (cDNA) synthesis and quantitative real-time polymerase chain reaction ... 29
Statistical analysis 29
Bioinformatic analysis 29
Results 30
Discussion 30
Conclusions 33
Conflicts of interest 33
Acknowledgements 33
Appendix A Supplementary data 33
References 33
5-Azacytidine restores interleukin 6-increased production in mesenchymal stromal cells from myelodysplastic patients 35
Introduction 36
Materials and methods 36
Patients and controls 36
MSC: MSC isolation and culture 36
Characterization of MSC 37
MSC cytokine production and 5-Azacytidine effect 37
Statistical analysis 37
Results 37
MDS patients and control subject demography 37
MDS-MSC presented different growth capacity and morphologic characteristics, compared to control-MSC 37
MSC immunophenotype and multipotenciality were similar between MDS and control subjects 38
MDS-MSC produced higher amounts of cytokines than controls 38
Treatment with azacytidine showed no cytotoxic effect on MSC 38
Azacytidine restores MDS-MSC IL-6 production 38
Discussion 39
Conflicts of interest 41
Acknowledgements 41
References 41
Desmopressin acetate use in von Willebrand’s disease: a survey on current practices in Brazil 43
Introduction 44
Methods 44
Study design, settings, inclusion and exclusion criteria 44
Variables 44
Statistical analysis 44
Results 45
Participants 45
Management of VWD and use of desmopressin acetate 45
Recognition of consensual indications and contra-indications of desmopressin acetate 46
Reasons for not using DDAVP while indicated 46
Comparisons between participants on the use of DDAVP 46
Evaluation of free responses 47
Discussion 47
Conclusion 49
Funding 49
Acknowledgements 49
Appendix A Supplementary data 49
References 49
Special article 50
Inclusion of molecular monitoring (BCR-ABL1) in the treatment of chronic myeloid leukemia in the Brazilian Public Health S... 50
Introduction 51
Methods 52
Panel of experts and consensus methodology 52
Questions to be answered 53
What is the importance of molecular monitoring with BCR-ABL in the treatment of CML patients? 53
Molecular monitoring is already reported in PCDT (Clinical Protocol and Therapeutic Guideline) of CML. In this scenario, h... 53
Molecular examination (quantification of BCR-ABL transcripts by RT-PCR) 54
Does SUS have technical and operational capacity to execute and implement BCR-ABL molecular monitoring, given the volume o... 54
What are the clinical and economic benefits of incorporating the BCR-ABL molecular test into SUS? 55
Clinical benefits 55
Economic benefits 55
What are the main strategies that could finance molecular testing in SUS? 55
Conclusion 56
Panel of specialists: crucial recommendations for 56
Funding 56
Conflicts of interest 56
References 56
Review article 58
Implications of perioperative allogeneic red blood cell transfusion on the immune-inflammatory response 58
Introduction 59
The normal immune response 59
Mechanisms of TRIM 59
TRIM mediated by allogeneic WBCs 60
TRIM mediated by soluble molecules 60
TRIM mediated by biologic mediators 61
Prognosis of colorectal cancer and blood transfusion 61
Final remarks and conclusions 62
Conflicts of interest 62
References 62
A review of hematopoietic stem cell transplantation for autoimmune diseases: multiple sclerosis, systemic sclerosis and Cr... 65
Introduction 65
Multiple sclerosis 66
Conventional therapeutic approaches for multiple sclerosis 67
Transplant in multiple sclerosis 67
Guidelines for transplantation in multiple sclerosis 73
Systemic sclerosis 73
Clinical presentation 73
Conventional therapeutic approaches 74
Hematopoietic stem cell transplantation 74
Guidelines for transplantation in systemic sclerosis 78
Crohn's disease 78
Hematopoietic stem cell therapy 81
Guidelines for transplantation in Crohn's disease 81
Conclusions 81
Conflicts of interest 82
Funding 82
References 82
Hemoglobinopathy and pediatrics in the time of COVID-19 87
Introduction 88
Objective 88
Methods 88
Search 88
Selection of scientific documents 88
Results 88
Discussion 97
Conflicts of interest 98
Acknowledgments 98
References 98
Case Report 101
Outcome of SARS-CoV-2 and immune thrombocytopenia in a pediatric patient 101
Introduction 101
Case report 102
Methods 102
Results and discussion 102
Conflict of interest 103
Acknowledgements 103
References 103
Acute chest syndrome and COVID-19 in sickle cell disease pediatric patients 104
Introduction 104
Patients presentation 104
Case 1 104
Case 2 105
Case 3 105
Discussion 107
Conclusion 108
References 108
Second COVID-19 infection in a patient with multiple myeloma in Brazil – reinfection or reactivation? 109
Introduction 109
Discussion 110
References 111
Letter to the Editor 112
COVID-19 and acute mesenteric ischemia: A review of literature 112
References 116
Erratum 117
Erratum on “Philadelphia-negative myeloproliferative neoplasms as disorders marked by cytokine modulation” 117

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