Hematology, Transfusion and Cell Therapy, Vol. 43, Issue 2, April-June 2021

Original Articles 1
Coping with COVID-19 pandemic in blood transfusion services in West Africa: the need to restrategize 1
Introduction 1
Methods 2
Results 2
COVID-19 pandemic in West Africa 2
Blood transfusion practice in West Africa 3
Discussion 4
Blood availability and safety during COVID-19 pandemic 4
Panacea 5
Inclusion of blood transfusion services in emergency response team 5
Establishment of blood management information system 5
Practice of social media medicine 5
Use of pharmacological agents 5
Issue of appointment letters and mobile blood drives 5
The use of “lowest hanging fruit” option 6
Conclusion 6
Author contributions 6
Conflicts of interest 6
References 6
Impact of the novel coronavirus disease and lockdown on the packed red blood cells inventory management: An experience fro... 8
Introduction 9
Methods 9
Various methods implemented for PRBC inventory management 9
Statistical analyses 10
Results 10
Discussion 12
Challenging differentiation between COVID-19 carriers and healthy blood donors 12
Risk of COVID-19 by blood transfusion 12
Policy after the donor reports the COVID-19 infection 12
Fall in demand for the blood components 12
Comparison with similar studies 12
Transfer of blood components between the various BTS 12
Transfusion requirements in COVID-19 patients 13
Role of the Hospital transfusion committee (HTC) 13
Push to the movement of patient blood management (PBM) 13
Preparedness plan for future epidemics 13
Conclusions 13
Author’s contribution 13
Conflicts of interest 13
Acknowledgments 13
Appendix A Supplementary data 13
References 13
Evaluation of Caripill™ as a component of platelet storage solution 15
Introduction 15
Materials and methods 16
Chemicals 16
Blood sampling 16
Isolation of platelets 16
Experimental design 16
Platelet functions 16
Platelet aggregation 16
ATP secretion 16
Platelet quality and metabolism 16
Lactate dehydrogenase (LDH, EC 1.1.1.27) 16
pH 16
Glucose 16
OS markers 16
Superoxides 16
Nitrites 16
Lipid peroxidation 17
Conjugate dienes 17
Thiobarbituric acid reactive substances (TBARS) 17
Protein oxidation 17
Protein carbonyls (PrC) 17
Protein sulfhydryls (P-SH) 17
Antioxidant defenses 17
Superoxide dismutase (SOD, EC 1.15.1.1) 17
Catalase (CAT, EC 1.11.1.6) 17
Total antioxidant capacity (TAC) — cupric ion reducing antioxidant capacity (CUPRAC) 17
Protein estimation 17
Statistical analyses 17
Results 17
Platelet function 17
Aggregation (without collagen) 17
Aggregation (with collagen) 17
ATP secretion 17
Platelet quality and metabolism 18
Lactate dehydrogenase (LDH) 18
pH 18
Glucose 18
OS markers 19
Free radicals 19
Superoxides 19
Nitrites 19
Lipid peroxidation 19
Conjugate dienes 19
Thiobarbituric acid reactive substances (TBARS) 19
Protein oxidation 19
Protein carbonyls (PrC) 19
Protein sulfhydryls (P-SH) 19
Antioxidant status 19
Superoxide dismutase (SOD) 19
Catalase (CAT) 19
Total antioxidant capacity (TAC) 19
Discussion 19
Conclusions 22
Funding 22
Conflicts of interest 22
Acknowledgement 22
References 22
Utility of BMI-1 and NANOG expression levels in survival prediction of pediatric acute lymphoblastic leukemia 23
Introduction 24
Materials and methods 24
Patient samples 24
Methods 24
PB mononuclear cells isolation 24
Quantitative real-time PCR (qRT-PCR) 25
Statistical analysis 25
Results 25
Bmi-1 and NANOG expression levels in ALL patients 25
Relationship between Bmi-1 and NANOG expression and the clinicopathological characteristics of pediatric ALL patients 25
Discussion 25
Author contributions 27
Conflicts of interest 27
References 27
Use of the reticulocyte channel warmed to 41°C of the XN-9000 analyzer in samples with the presence of cold agglutinins 29
Introduction 30
Materials and methods 30
Instrumental characteristics 30
Blood samples 31
Study design 31
Statistical analysis 31
Results 31
Discussion 34
Conclusions 36
Conflicts of interest 36
References 36
Generation of hematopoietic stem/progenitor cells with sickle cell mutation from induced pluripotent stem cell in serum-fr... 38
Introduction 39
Material and methods 39
Ethical aspects and iPSC line 39
Hematopoietic and erythroid differentiation 39
Immunophenotypic profile determination by flow cytometry 39
Results 40
Characterization of iPSCs derived from CD34+ peripheral blood mononuclear cells 40
Generation of hematopoietic stem/progenitor cells in a feeder-free and serum-free culture condition 40
Characterization of erythroid progenitor cells 42
Discussion 42
Conclusions 45
Data availability 45
Conflicts of interest 45
Acknowledgments 45
Appendix A Supplementary data 45
References 45
Polymorphisms in the heme oxygenase-1 and bone morphogenetic protein receptor type 1b genes and estimated glomerular filtr... 47
Introduction 48
Methods 48
Results 49
Clinical data of patients 49
Allelic and genotypic distributions of HMOX1 promoter polymorphisms 49
Allelic and genotypic distributions of BMPR1B polymorphisms 49
Discussion 49
Funding 51
Conflicts of interest 51
References 51
Causes of death and mortality trends related to hemophilia in Brazil, 1999 to 2016 53
Introduction 54
Material and methods 54
Results 55
Discussion 57
Conflicts of interest 59
References 59
Comparison of abo antibody levels in apheresis platelets suspended in platelet additive solution and plasma 61
Background 62
Materials and methods 62
Study population 62
Titration procedure 62
Statistical methods 62
Results 62
Discussion 62
Conclusion 65
Ethical approval 65
Conflicts of interest 65
References 65
Review Articles 67
Do cytomegalovirus infections affect the daratumumab treatment course in multiple myeloma patients? – Literature review 67
Introduction 68
Materials and methods 68
Results and discussion 69
Conclusion 71
Conflicts of interest 71
References 71
Recommendations for the management of cardiovascular risk in patients with chronic myeloid leukemia on tyrosine kinase inh... 73
Introduction 74
Risk assessment and stratification 75
Very high risk 75
High risk 75
Intermediate risk 77
Low risk 77
Treatment 77
Hypertension control 77
Diabetes control 77
Smoking control 78
LDL-c control 78
Monitoring 79
Final considerations 80
Funding source 80
Conflicts of interest 80
Acknowledgements 80
References 80
Convalescent plasma therapy - a silver lining for COVID-19 management? 83
Introduction 83
Historical evidence 84
Convalescent plasma therapy in COVID-19 85
Discussion 89
Conflict of interest 90
References 90
Special Article 94
Suggested guidelines for convalescent plasma therapy for the treatment of COVID-19 94
Conflicts of interest 95
References 95
Case Reports 96
COVID-19 and bleeding at unusual locations: Report of four cases 96
Introduction 96
Case presentation 96
Discussion 98
Funding 99
Conflicts of interest 99
References 99
COVID-19 and adult acute lymphoblastic leukemia: Presentation and management 101
Introduction 101
Case report 101
Discussion 102
Conclusion 103
Conflicts of interest 103
References 103
Ascites as a presenting sign of multicentric mixed-type Castleman disease variant of POEMS syndrome 104
Introduction 104
Case presentation 104
Discussion 106
Conclusion 107
Consent 107
Author’s contribution 107
Conflicts of interest 107
References 107
Images in Clinical Hematology 108
Erythropoietin-producing clear cell renal cell carcinoma associated with secondary polycythemia 108
Conflicts of interest 109
References 109

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