Hematology, Transfusion and Cell Therapy, Vol. 43, Issue 2, April-June 2021

Original Articles	1
Coping with COVID-19 pandemic in blood transfusion services in West Africa: the need to restrategize	1
Introduction	1
Methods	2
Results	2
COVID-19 pandemic in West Africa	2
Blood transfusion practice in West Africa	3
Discussion	4
Blood availability and safety during COVID-19 pandemic	4
Panacea	5
Inclusion of blood transfusion services in emergency response team	5
Establishment of blood management information system	5
Practice of social media medicine	5
Use of pharmacological agents	5
Issue of appointment letters and mobile blood drives	5
The use of “lowest hanging fruit” option	6
Conclusion	6
Author contributions	6
Conflicts of interest	6
References	6
Impact of the novel coronavirus disease and lockdown on the packed red blood cells inventory management: An experience fro...	8
Introduction	9
Methods	9
Various methods implemented for PRBC inventory management	9
Statistical analyses	10
Results	10
Discussion	12
Challenging differentiation between COVID-19 carriers and healthy blood donors	12
Risk of COVID-19 by blood transfusion	12
Policy after the donor reports the COVID-19 infection	12
Fall in demand for the blood components	12
Comparison with similar studies	12
Transfer of blood components between the various BTS	12
Transfusion requirements in COVID-19 patients	13
Role of the Hospital transfusion committee (HTC)	13
Push to the movement of patient blood management (PBM)	13
Preparedness plan for future epidemics	13
Conclusions	13
Author’s contribution	13
Conflicts of interest	13
Acknowledgments	13
Appendix A Supplementary data	13
References	13
Evaluation of Caripill™ as a component of platelet storage solution	15
Introduction	15
Materials and methods	16
Chemicals	16
Blood sampling	16
Isolation of platelets	16
Experimental design	16
Platelet functions	16
Platelet aggregation	16
ATP secretion	16
Platelet quality and metabolism	16
Lactate dehydrogenase (LDH, EC 1.1.1.27)	16
pH	16
Glucose	16
OS markers	16
Superoxides	16
Nitrites	16
Lipid peroxidation	17
Conjugate dienes	17
Thiobarbituric acid reactive substances (TBARS)	17
Protein oxidation	17
Protein carbonyls (PrC)	17
Protein sulfhydryls (P-SH)	17
Antioxidant defenses	17
Superoxide dismutase (SOD, EC 1.15.1.1)	17
Catalase (CAT, EC 1.11.1.6)	17
Total antioxidant capacity (TAC) — cupric ion reducing antioxidant capacity (CUPRAC)	17
Protein estimation	17
Statistical analyses	17
Results	17
Platelet function	17
Aggregation (without collagen)	17
Aggregation (with collagen)	17
ATP secretion	17
Platelet quality and metabolism	18
Lactate dehydrogenase (LDH)	18
pH	18
Glucose	18
OS markers	19
Free radicals	19
Superoxides	19
Nitrites	19
Lipid peroxidation	19
Conjugate dienes	19
Thiobarbituric acid reactive substances (TBARS)	19
Protein oxidation	19
Protein carbonyls (PrC)	19
Protein sulfhydryls (P-SH)	19
Antioxidant status	19
Superoxide dismutase (SOD)	19
Catalase (CAT)	19
Total antioxidant capacity (TAC)	19
Discussion	19
Conclusions	22
Funding	22
Conflicts of interest	22
Acknowledgement	22
References	22
Utility of BMI-1 and NANOG expression levels in survival prediction of pediatric acute lymphoblastic leukemia	23
Introduction	24
Materials and methods	24
Patient samples	24
Methods	24
PB mononuclear cells isolation	24
Quantitative real-time PCR (qRT-PCR)	25
Statistical analysis	25
Results	25
Bmi-1 and NANOG expression levels in ALL patients	25
Relationship between Bmi-1 and NANOG expression and the clinicopathological characteristics of pediatric ALL patients	25
Discussion	25
Author contributions	27
Conflicts of interest	27
References	27
Use of the reticulocyte channel warmed to 41°C of the XN-9000 analyzer in samples with the presence of cold agglutinins	29
Introduction	30
Materials and methods	30
Instrumental characteristics	30
Blood samples	31
Study design	31
Statistical analysis	31
Results	31
Discussion	34
Conclusions	36
Conflicts of interest	36
References	36
Generation of hematopoietic stem/progenitor cells with sickle cell mutation from induced pluripotent stem cell in serum-fr...	38
Introduction	39
Material and methods	39
Ethical aspects and iPSC line	39
Hematopoietic and erythroid differentiation	39
Immunophenotypic profile determination by flow cytometry	39
Results	40
Characterization of iPSCs derived from CD34+ peripheral blood mononuclear cells	40
Generation of hematopoietic stem/progenitor cells in a feeder-free and serum-free culture condition	40
Characterization of erythroid progenitor cells	42
Discussion	42
Conclusions	45
Data availability	45
Conflicts of interest	45
Acknowledgments	45
Appendix A Supplementary data	45
References	45
Polymorphisms in the heme oxygenase-1 and bone morphogenetic protein receptor type 1b genes and estimated glomerular filtr...	47
Introduction	48
Methods	48
Results	49
Clinical data of patients	49
Allelic and genotypic distributions of HMOX1 promoter polymorphisms	49
Allelic and genotypic distributions of BMPR1B polymorphisms	49
Discussion	49
Funding	51
Conflicts of interest	51
References	51
Causes of death and mortality trends related to hemophilia in Brazil, 1999 to 2016	53
Introduction	54
Material and methods	54
Results	55
Discussion	57
Conflicts of interest	59
References	59
Comparison of abo antibody levels in apheresis platelets suspended in platelet additive solution and plasma	61
Background	62
Materials and methods	62
Study population	62
Titration procedure	62
Statistical methods	62
Results	62
Discussion	62
Conclusion	65
Ethical approval	65
Conflicts of interest	65
References	65
Review Articles	67
Do cytomegalovirus infections affect the daratumumab treatment course in multiple myeloma patients? – Literature review	67
Introduction	68
Materials and methods	68
Results and discussion	69
Conclusion	71
Conflicts of interest	71
References	71
Recommendations for the management of cardiovascular risk in patients with chronic myeloid leukemia on tyrosine kinase inh...	73
Introduction	74
Risk assessment and stratification	75
Very high risk	75
High risk	75
Intermediate risk	77
Low risk	77
Treatment	77
Hypertension control	77
Diabetes control	77
Smoking control	78
LDL-c control	78
Monitoring	79
Final considerations	80
Funding source	80
Conflicts of interest	80
Acknowledgements	80
References	80
Convalescent plasma therapy - a silver lining for COVID-19 management?	83
Introduction	83
Historical evidence	84
Convalescent plasma therapy in COVID-19	85
Discussion	89
Conflict of interest	90
References	90
Special Article	94
Suggested guidelines for convalescent plasma therapy for the treatment of COVID-19	94
Conflicts of interest	95
References	95
Case Reports	96
COVID-19 and bleeding at unusual locations: Report of four cases	96
Introduction	96
Case presentation	96
Discussion	98
Funding	99
Conflicts of interest	99
References	99
COVID-19 and adult acute lymphoblastic leukemia: Presentation and management	101
Introduction	101
Case report	101
Discussion	102
Conclusion	103
Conflicts of interest	103
References	103
Ascites as a presenting sign of multicentric mixed-type Castleman disease variant of POEMS syndrome	104
Introduction	104
Case presentation	104
Discussion	106
Conclusion	107
Consent	107
Author’s contribution	107
Conflicts of interest	107
References	107
Images in Clinical Hematology	108
Erythropoietin-producing clear cell renal cell carcinoma associated with secondary polycythemia	108
Conflicts of interest	109
References	109

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